Article
An archaeal family-B DNA polymerase variant able to replicate past DNA damage: occurrence of replicative and translesion synthesis polymerases within the B family.
Nucleic acids research - 1 Sept 2014
Jozwiakowski Stanislaw K, Keith Brian J, Gilroy Louise, Doherty Aidan J, Connolly Bernard A
Abstract excerpt
A mutant of the high fidelity family-B DNA polymerase from the archaeon Thermococcus gorgonarius (Tgo-Pol), able to replicate past DNA lesions, is described. Gain of function requires replacement of the three amino acid loop region in the fingers domain of Tgo-Pol with a longer version, found naturally in eukaryotic Pol ζ (a family-B translesion synthesis polymerase). Inactivation of the 3'-5' proof-reading...
Topics
- Archaeal Proteins
- Base Pair Mismatch
- DNA Damage
- DNA Polymerase II
- DNA Primers
- DNA Replication
- DNA-Directed DNA Polymerase
- Escherichia coli
- Mutation
- Pyrimidine Dimers
- Templates, Genetic
- Thermococcus