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Plasmid-based lacZalpha assay for DNA polymerase fidelity: application to archaeal family-B DNA polymerase.

Nucleic acids research - 1 Aug 2009

Jozwiakowski Stanislaw K, Connolly Bernard A

Abstract excerpt

The preparation of a gapped pUC18 derivative, containing the lacZalpha reporter gene in the single-stranded region, is described. Gapping is achieved by flanking the lacZalpha gene with sites for two related nicking endonucleases, enabling the excision of either the coding or non-coding strand. However, the excised strand remains annealed to the plasmid through non-covalent Watson-Crick base-pairing; its removal,...

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Identifiers

PMID: 19515939
DOI: 10.1093/nar/gkp494
PMCID: PMC2731893

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