Article
Genome-wide identification of transcript start and end sites by transcript isoform sequencing.
Nature protocols - 1 Jul 2014
Pelechano Vicent, Wei Wu, Jakob Petra, Steinmetz Lars M
Abstract excerpt
Hundreds of transcript isoforms with varying boundaries and alternative regulatory signals are transcribed from the genome, even in a genetically homogeneous population of cells. To study this transcriptional heterogeneity, we developed transcript isoform sequencing (TIF-seq), a method that allows the genome-wide profiling of full-length transcript isoforms defined by their exact 5' and 3' boundaries. TIF-seq...
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