Article

A systematic benchmark of Nanopore long read RNA sequencing for transcript level analysis in human cell lines

2021-04-22

Abstract excerpt

The human genome contains more than 200,000 gene isoforms. However, different isoforms can be highly similar, and with an average length of 1.5kb remain difficult to study with short read sequencing. To systematically evaluate the ability to study the transcriptome at a resolution of individual isoforms we profiled 5 human cell lines with short read cDNA sequencing and Nanopore long read direct RNA, amplification-...

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Literature Corpus work: c02bf1f2-b605-5685-adba-292db0f7c767
DOI: 10.1101/2021.04.21.440736

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