Molecular analysis of spontaneous mutations at the gpt locus in Chinese hamster ovary (AS52) cells.

doi:10.1016/0165-1110(89)90028-6

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Article

Molecular analysis of spontaneous mutations at the gpt locus in Chinese hamster ovary (AS52) cells.

Mutation research - 1 Jan 2000

Tindall K R, Stankowski L F

Abstract excerpt

AS52 cells are Chinese hamster ovary (CHO) cells that carry a single functional copy of the bacterial gpt gene and allow the isolation of 6-thioguanine-resistant (6TGr)mutants arising from mutation at the chromosally integrated gpt locus. The gpt locus in AS52 cells is extremely stable, giving ri...

Topics

Animals
Base Sequence
Blotting, Southern
Cell Line
Cricetinae
Cricetulus
Gene Amplification
Hypoxanthine Phosphoribosyltransferase
Mutagenicity Tests
Mutation
Pentosyltransferases
Thioguanine

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