Article
Polymerase chain reaction-based comprehensive procedure for the analysis of the mutation spectrum at the hypoxanthine-guanine phosphoribosyltransferase locus in Chinese hamster cells.
Environmental and molecular mutagenesis - 1 Jan 1992
Yu Y J, Xu Z, Gibbs R A, Hsie A W
Abstract excerpt
We have established a comprehensive procedure based on the polymerase chain reaction (PCR) to analyze the molecular spectrum of mutations at the hypoxanthine-guanine phosphoribosyltransferase (hprt) locus in Chinese hamster cells. The procedure includes direct sequencing of PCR-amplified hprt cDNA for locating point mutations in the expressed coding sequences, multiplex PCR amplification of the hprt exons for...
Topics
- Animals
- Base Sequence
- CHO Cells
- Cricetinae
- DNA
- Exons
- Hypoxanthine Phosphoribosyltransferase
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction