Article
Genome wide spontaneous mutation in human cells determined by the spectrum of mutations in hprt cDNA genes.
Mutagenesis - 1 May 1993
Lichtenauer-Kaligis E G, Thijssen J, den Dulk H, van de Putte P, Tasseron-de Jong J G, Giphart-Gassler M
Abstract excerpt
We have studied spontaneous mutagenesis in five hprt cDNA genes integrated at five different genomic positions in a human lymphoblastoid cell line (TK6). The spectra of 40 mutants from each position were combined to obtain a mutation spectrum of the overall genome. This collection of mutants was...
Topics
- Adenine Phosphoribosyltransferase
- Animals
- Base Composition
- Base Sequence
- Cell Line
- DNA
- DNA Replication
- Genome, Human
- Humans
- Hypoxanthine Phosphoribosyltransferase
- Models, Genetic
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Point Mutation
- Sequence Deletion