Article
Molecular nature of spontaneous mutations at the hypoxanthine-guanine phosphoribosyltransferase (hprt) locus in Chinese hamster ovary cells.
Environmental and molecular mutagenesis - 1 Jan 1995
Xu Z, Yu Y, Schwartz J L, Meltz M L, Hsie A W
Abstract excerpt
The hypoxanthine-guanine phosphoribosyltransferase (hprt) locus has been widely used as a selectable genetic marker for studies of mammalian cell mutagenesis. We report here the spontaneous mutation spectrum at the hprt locus in 64 independently isolated mutants of Chinese hamster ovary (CHO) cel...
Topics
- Animals
- Base Sequence
- CHO Cells
- Cells, Cultured
- Cricetinae
- Cricetulus
- DNA Primers
- DNA, Complementary
- Exons
- Frameshift Mutation
- Gene Deletion
- Genes, araC
- Hypoxanthine Phosphoribosyltransferase
- Molecular Sequence Data
- Multigene Family
- Mutation
- Polymerase Chain Reaction
- RNA Splicing