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Article

MODEST: a web-based design tool for oligonucleotide-mediated genome engineering and recombineering.

Nucleic acids research - 1 Jul 2014

Bonde Mads T, Klausen Michael S, Anderson Mads V, Wallin Annika I N, Wang Harris H, Sommer Morten O A

Abstract excerpt

Recombineering and multiplex automated genome engineering (MAGE) offer the possibility to rapidly modify multiple genomic or plasmid sites at high efficiencies. This enables efficient creation of genetic variants including both single mutants with specifically targeted modifications as well as combinatorial cell libraries. Manual design of oligonucleotides for these approaches can be tedious, time-consuming, and...

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PMID: 24838561
DOI: 10.1093/nar/gku428
PMCID: PMC4086063

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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