Article
The transition to an elongation complex by T7 RNA polymerase is a multistep process.
The Journal of biological chemistry - 3 Aug 2007
Bandwar Rajiv P, Ma Na, Emanuel Steven A, Anikin Michael, Vassylyev Dmitry G, Patel Smita S, McAllister William T
Abstract excerpt
During the transition from an initiation complex to an elongation complex (EC), T7 RNA polymerase undergoes major conformational changes that involve reorientation of a "core" subdomain as a rigid body and extensive refolding of other elements in the 266 residue N-terminal domain. The pathway and timing of these events is poorly understood. To examine this, we introduced proline residues into regions of the...
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