Article
A highly sensitive and nonradioactive mutation detection method based on vertical gradient temperature single-strand conformation polymorphism.
Electrophoresis - 1 Aug 2001
Razzaghi H, Kamboh M I
Abstract excerpt
The single-strand conformation polymorphism (SSCP) method is widely used for mutation detection. The sensitivity of the method depends on several factors, most importantly on the temperature at which electrophoresis of single-stranded DNA (ssDNA) takes place. The temperature has a profound effect on the folded conformation of ssDNA. The temperature factor is predominantly determined empirically in conventional...
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