Flow cytometric quantification of mutant T cells with altered expression of the T-cell receptor: detecting somatic mutants in humans and mice.

Abstract excerpt

Spontaneously generated mutant T cells defective in T-cell receptor (TCR) gene expression are detectable at the frequency of 2×10(-4) in vivo, and the mutant fractions are dose dependently increased by exposure to genotoxic agents such as ionizing radiation. Mutant cells with altered expression of TCRα or -β among CD4(+) T cells can be detected as CD3(-)/CD4(+) cells by two-color flow cytometry using anti-CD3 and...

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