Article
Development of a flow-cytometric HLA-A locus mutation assay for human peripheral blood lymphocytes.
Mutation research - 1 Aug 1992
Kushiro J, Hirai Y, Kusunoki Y, Kyoizumi S, Kodama Y, Wakisaka A, Jeffreys A, Cologne J B, Dohi K, Nakamura N
Abstract excerpt
A flow-cytometric technique was developed to measure the frequency of variant lymphocytes lacking expression of HLA-A2 or A24 allele products among donors heterozygous for HLA-A2 or A24. It was found that the variant frequency of lymphocytes in peripheral blood was of the order of 10(-4) and increased with donor age. Molecular analyses of mutant clones revealed that about one-third were derived from somatic...
Topics
- Adult
- Aged
- Blotting, Southern
- Carcinogenicity Tests
- Cells, Cultured
- Chromosome Aberrations
- Flow Cytometry
- Genes, MHC Class I
- HLA-A Antigens
- HLA-A2 Antigen
- HLA-A24 Antigen