Article
Flow cytometric measurement of mutant T cells with altered expression of TCR: detecting somatic mutations in humans and mice.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2005
Kyoizumi Seishi, Kusunoki Yoichiro, Hayashi Tomonori
Abstract excerpt
Spontaneously generated mutant T cells defective in T-cell receptor (TCR) gene expression are detectable at the frequency of 10(-4) in vivo, and the mutant fractions are dose-dependently increased by exposure to genotoxic substances such as ionizing radiation. Mutant cells with altered expression of TCR-alpha or -beta among CD4+ T cells can be detected as CD3-/CD4+ cells by two-color flow cytometry using anti-CD3...
Topics
- Animals
- CD4-Positive T-Lymphocytes
- Flow Cytometry
- Humans
- Mice
- Mutagenicity Tests
- Mutation
- Receptors, Antigen, T-Cell