Southern-blot analyses of human T-lymphocyte mutants induced in vitro by gamma-irradiation.

doi:10.1016/0165-1218(90)90018-w

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Article

Southern-blot analyses of human T-lymphocyte mutants induced in vitro by gamma-irradiation.

Mutation research - 1 Feb 1990

O'Neill J P, Hunter T C, Sullivan L M, Nicklas J A, Albertini R J

Abstract excerpt

G0 phase cultures of human peripheral blood T-lymphocytes from a single individual were exposed to 300 rad of gamma-irradiation from a 137Cs source and cultured in vitro for 8 days to allow phenotypic expression. Thioguanine-resistant (TGr) mutants were isolated by a cell cloning assay in microtiter plates. These mutants were studied by Southern blot analysis to define the gross structural alterations in the...

Topics

Blotting, Southern
Cells, Cultured
Clone Cells
DNA Probes
DNA Restriction Enzymes
Drug Resistance
Gamma Rays
Gene Rearrangement
Humans
Hypoxanthine Phosphoribosyltransferase
Interphase

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Male

Mutation

Radiation, Ionizing

Receptors, Antigen, T-Cell

T-Lymphocytes

Thioguanine

Source

Open in PubMed

Identifiers

PMID: 2153929
DOI: 10.1016/0165-1218(90)90018-w

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2153929-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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