Article
Development of a base editor for protein evolution via in situ mutation in vivo.
Nucleic acids research - 20 Sept 2021
Hao Wenliang, Cui Wenjing, Cheng Zhongyi, Han Laichuang, Suo Feiya, Liu Zhongmei, Zhou Li, Zhou Zhemin
Abstract excerpt
Protein evolution has significantly enhanced the development of life science. However, it is difficult to achieve in vitro evolution of some special proteins because of difficulties with heterologous expression, purification, and function detection. To achieve protein evolution via in situ mutation in vivo, we developed a base editor by fusing nCas with a cytidine deaminase in Bacillus subtilis through genome...
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