Article
A simple and reproducible method for directed evolution: combination of random mutation with dITP and DNA fragmentation with endonuclease V.
Molecular biotechnology - 1 Jan 2013
Wang Zun, Wang Hai-Yan, Feng Hong
Abstract excerpt
An alternative method to combine mutagenesis PCR with dITP and fragmentation by endonuclease V for directed evolution was developed. In comparison to the routine protocol for directed evolution, dITP was used as mutation reagent in the mutagenesis PCR. Subsequently, the incorporated dITP in the PCR products could represent as being the target of endonuclease V. Finally, the mutated dsDNA was fragmented by...
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