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Article

The accurate replacement of long genome region more than several hundreds kilobases in Bacillus subtilis.

Genes & genetic systems - 1 Feb 2007

Liu Shenghao, Endo Keiji, Ara Katsutoshi, Ozaki Katsuya, Ogasawara Naotake

Abstract excerpt

Competent cell transformation with DNA obtained by the gentle lysis of protoplasts (LP transformation) was used to replace a large genomic region in this study. Discontinuity was detected in the replacement of the donor region tested, probably due to multiple crossover events involving a single donor genome fragment. To overcome discontinuous replacement, we inverted the genomic region to be replaced in the donor...

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PMID: 17396016
DOI: 10.1266/ggs.82.9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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