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Article

Direct Isolation of Seamless Mutant Bacterial Artificial Chromosomes.

Current protocols in molecular biology - 3 Apr 2017

Lyozin George T, Kosaka Yasuhiro, Bhattacharje Gourab, Yost H Joseph, Brunelli Luca

Abstract excerpt

Seamless (i.e., without unwanted DNA sequences) mutant bacterial artificial chromosomes (BACs) generated via recombination-mediated genetic engineering (recombineering) are better suited to study gene function compared to complementary DNA (cDNA) because they contain only the specific mutation and provide all the regulatory sequences required for in vivo gene expression. However, precisely mutated BACs are...

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Identifiers

PMID: 28369677
DOI: 10.1002/cpmb.34

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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