Article
Multiplexed in-situ mutagenesis driven by a dCas12a-based dual-function base editor.
Nucleic acids research - 8 May 2024
Wu Yaokang, Li Yang, Liu Yanfeng, Xiu Xiang, Liu Jiaheng, Zhang Linpei, Li Jianghua, Du Guocheng, Lv Xueqin, Chen Jian, Ledesma-Amaro Rodrigo, Liu Long
Abstract excerpt
Mutagenesis driving genetic diversity is vital for understanding and engineering biological systems. However, the lack of effective methods to generate in-situ mutagenesis in multiple genomic loci combinatorially limits the study of complex biological functions. Here, we design and construct MultiduBE, a dCas12a-based multiplexed dual-function base editor, in an all-in-one plasmid for performing combinatorial...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.