Efficient and precise construction of markerless manipulations in the Bacillus subtilis genome.

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Article

Efficient and precise construction of markerless manipulations in the Bacillus subtilis genome.

Journal of microbiology and biotechnology - 1 Jan 2010

Yu Haojie, Yan Xin, Shen Weiliang, Shen Yujia, Zhang Ji, Li Shunpeng

Abstract excerpt

We have developed an efficient and precise method for genome manipulations in Bacillus subtilis that allows rapid alteration of gene sequence or multiple gene sequences without altering the chromosome in any other way. In our approach, the Escherichia coli toxin gene mazF, which was used as a counter-selectable marker, was placed under the control of a xylose-inducible expression system and associated with an...

Topics

Bacillus subtilis
DNA-Binding Proteins
Endoribonucleases
Escherichia coli Proteins
Gene Targeting
Genome, Bacterial
Mutation
Recombination, Genetic

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Open in PubMed

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PMID: 20134232

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