Article
Function of the N-terminal half of RepA in activation of Rts1 ori.
Journal of bacteriology - 1 Nov 1992
Terawaki Y, Itoh Y, Zeng H, Hayashi T, Tabuchi A
Abstract excerpt
The RepA protein of the Rts1 plasmid, consisting of 288 amino acids, is a trans-acting protein essential for replication. A mutant repA gene, repA delta C143, carrying a deletion that removed the 143 C-terminal amino acids of RepA, could transform, but at a low frequency, an Escherichia coli polA strain, JG112, when repA delta C143 was cloned into pBR322 with Rts1 ori in the natural configuration. The...
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