Article
Function of the N-terminal half of RepA in activation of Rts1 ori.
Journal of bacteriology - 1 Nov 1992
Terawaki Y, Itoh Y, Zeng H, Hayashi T, Tabuchi A
Abstract excerpt
The RepA protein of the Rts1 plasmid, consisting of 288 amino acids, is a trans-acting protein essential for replication. A mutant repA gene, repA delta C143, carrying a deletion that removed the 143 C-terminal amino acids of RepA, could transform, but at a low frequency, an Escherichia coli polA...
Topics
- Bacterial Proteins
- DNA Helicases
- DNA Mutational Analysis
- DNA Replication
- DNA-Binding Proteins
- Escherichia coli
- Lac Operon
- Mutagenesis, Site-Directed
- Phenotype
- Plasmids
- Promoter Regions, Genetic
- Proteins