Article
Modulation of pPS10 host range by plasmid-encoded RepA initiator protein.
Journal of bacteriology - 1 Feb 2003
Maestro Beatriz, Sanz Jesús M, Díaz-Orejas Ramón, Fernández-Tresguerres Elena
Abstract excerpt
We report here the isolation and analysis of novel repA host range mutants of pPS10, a plasmid originally found in Pseudomonas savastanoi. Upon hydroxylamine treatment, five plasmid mutants were selected for their establishment in Escherichia coli at 37 degrees C, a temperature at which the wild-type form cannot be established. The mutations were located in different functional regions of the plasmid RepA...
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