Next generation sequencing to determine HLA class II genotypes in a cohort of hematopoietic cell transplant patients and donors.

doi:10.1016/j.humimm.2014.08.206

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Article

Next generation sequencing to determine HLA class II genotypes in a cohort of hematopoietic cell transplant patients and donors.

Human immunology - 1 Oct 2014

Smith Anajane G, Pyo Chul-Woo, Nelson Wyatt, Gow Edward, Wang Ruihan, Shen Shu, Sprague Maggie, Pereira Shalini E, Geraghty Daniel E, Hansen John A

Abstract excerpt

Current high-resolution HLA typing technologies frequently produce ambiguous results that mandate extended testing prior to reporting. Through multiplex sequencing of individual amplicons from many individuals at multiple loci, next generation sequencing (NGS) promises to eliminate heterozygote ambiguities and extend the breadth of genetic data acquired with little additional effort. We report here on assessment...

Topics

Cohort Studies
Exons
Genotype
HLA-DP alpha-Chains
HLA-DP beta-Chains
HLA-DQ alpha-Chains
HLA-DQ beta-Chains
HLA-DR Antigens
Hematopoietic Stem Cell Transplantation
High-Throughput Nucleotide Sequencing

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Histocompatibility Antigens Class II

Histocompatibility Testing

Humans

Living Donors

Reproducibility of Results

Software

Source

Open in PubMed

Identifiers

PMID: 25167774
DOI: 10.1016/j.humimm.2014.08.206

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-25167774-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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