Article
Comprehensive interrogation of a minimalist synthetic CDR-H3 library and its ability to generate antibodies with therapeutic potential.
Journal of molecular biology - 27 May 2013
Mahon Ciara M, Lambert Matthew A, Glanville Jacob, Wade Jason M, Fennell Brian J, Krebs Mark R, Armellino Douglas, Yang Sharon, Liu Xuemei, O'Sullivan Cliona M, Autin Benedicte, Oficjalska Katarzyna, Bloom Laird, Paulsen Janet, Gill Davinder, Damelin Marc, Cunningham Orla, Finlay William J J
Abstract excerpt
We have generated large libraries of single-chain Fv antibody fragments (>10(10) transformants) containing unbiased amino acid diversity that is restricted to the central combining site of the stable, well-expressed DP47 and DPK22 germline V-genes. Library WySH2A was constructed to examine the potential for synthetic complementarity-determining region (CDR)-H3 diversity to act as the lone source of binding...
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