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Article

A general method for greatly improving the affinity of antibodies by using combinatorial libraries.

Proceedings of the National Academy of Sciences of the United States of America - 14 Jun 2005

Rajpal Arvind, Beyaz Nurten, Haber Lauric, Cappuccilli Guido, Yee Helena, Bhatt Ramesh R, Takeuchi Toshihiko, Lerner Richard A, Crea Roberto

Abstract excerpt

Look-through mutagenesis (LTM) is a multidimensional mutagenesis method that simultaneously assesses and optimizes combinatorial mutations of selected amino acids. The process focuses on a precise distribution within one or more complementarity determining region (CDR) domains and explores the sy...

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Source

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Identifiers

PMID: 15939870
DOI: 10.1073/pnas.0503543102
PMCID: PMC1143585

Genes

TNF

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-15939870-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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