Article
Creation of the large and highly functional synthetic repertoire of human VH and Vκ domain antibodies.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012
Ignatovich Olga, Jespers Laurent, Tomlinson Ian M, de Wildt Ruud M T
Abstract excerpt
This protocol describes a method for creation of a highly diverse and functional synthetic phage-displayed repertoire of fully human domain antibodies (dAbs). The repertoire is based on two human frameworks (one VH and one Vκ) that express well in bacteria and are frequently used in human antibodies. To achieve this, we first build dAb libraries, containing full synthetic diversity at key positions in the...
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