Article
Engineering visual arrestin-1 with special functional characteristics.
The Journal of biological chemistry - 1 Feb 2013
Vishnivetskiy Sergey A, Chen Qiuyan, Palazzo Maria C, Brooks Evan K, Altenbach Christian, Iverson Tina M, Hubbell Wayne L, Gurevich Vsevolod V
Abstract excerpt
Arrestin-1 preferentially binds active phosphorylated rhodopsin. Previously, a mutant with enhanced binding to unphosphorylated active rhodopsin (Rh*) was shown to partially compensate for lack of rhodopsin phosphorylation in vivo. Here we showed that reengineering of the receptor binding surface of arrestin-1 further improves the binding to Rh* while preserving protein stability. In mammals, arrestin-1 readily...
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