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Article

Preparation and characterization of a monoclonal antibody against the refolded and functional extracellular domain of rat P2X4 receptor.

Journal of biochemistry - 1 Mar 2013

Igawa Tatsuhiro, Higashi Sadayuki, Abe Yoshito, Ohkuri Takatoshi, Tanaka Hiroyuki, Morimoto Satoshi, Yamashita Tomohiro, Tsuda Makoto, Inoue Kazuhide, Ueda Tadashi

Abstract excerpt

The P2X4 purinergic receptor is a key molecule in neuropathic pain, particularly in the allodynia after peripheral nerve injury. We therefore sought to establish an anti-P2X4 receptor monoclonal antibody that would be useful for detection and characterization of the P2X4 receptor. We first prepared the refolded extracellular domain of the rat P2X4 receptor expressed in Escherichia coli. Then, we established a...

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PMID: 23220420
DOI: 10.1093/jb/mvs143

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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