Article
Improvement of the affinity of an anti-rat P2X4 receptor antibody by introducing electrostatic interactions.
Scientific reports - 7 Jan 2022
Shinozaki Chinatsu, Kohno Keita, Shiroishi Mitsunori, Takahashi Daisuke, Yoshikawa Yu, Abe Yoshito, Hamase Kenji, Nakakido Makoto, Tsumoto Kohei, Inoue Kazuhide, Tsuda Makoto, Ueda Tadashi
Abstract excerpt
We have recently developed a mouse monoclonal antibody (12-10H) binding to the head domain region in rat P2X4 receptor (rP2X4R, which is crucial for the pathogenesis of neuropathic pain) expressed on the cell with the highest binding affinity (KD = 20 nM). However, the 12-10H antibody failed to detect endogenously expressed P2X4Rs in microglia isolated from the spinal cord of rats whose spinal nerves were...
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