Article
Electrochemiluminescent assay for detection of extremely rare mutations based on ligase reaction and bead enrichment.
Analytical biochemistry - 1 Mar 2013
Cheng Changming, Wang Jie, Yang Chao, Zhou Yin, Chen Juan, Zhang Jie, Jia Nengqin, Cao Hongmei, Zhao Guoping
Abstract excerpt
The detection of rare mutations is particularly essential in many areas of biomedical research. Here, we report an ultrasensitive method to detect extremely rare point mutations based on electrochemiluminescent assay. The point mutation among large excess wild-type alleles is exclusively amplified through ligase detection reaction. The products corresponding to the amplification of mutant alleles are selectively...
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