Ferrocene-labeled and purification-free electrochemical biosensor based on ligase chain reaction for ultrasensitive single nucleotide polymorphism detection.

Abstract excerpt

Single nucleotide polymorphisms (SNPs) are crucial during the early diagnosis of a given disease as well as in evaluating their response to certain drugs. Thus, this study sought the development of ferrocene (Fc)-labeled electrochemical biosensor for SNP detection. This proposed system involves the ligation of four short probes (e.g., A, B, A', and B', where B' is labeled with an Fc-tag) in the presence of target...

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• Biosensing Techniques
• DNA
• DNA Ligases
• Electrochemical Techniques
• Ferrous Compounds