Article
A simple and ultrasensitive fluorescence assay for single-nucleotide polymorphism.
Analytical and bioanalytical chemistry - 1 May 2018
Ma Qian, Gao Zhiqiang
Abstract excerpt
In this report, a simple, label-free and highly efficient nucleic acid amplification technique is developed for ultrasensitive detection of single-nucleotide polymorphism (SNP). Briefly, a designed padlock probe is first circularized by a DNA ligase when it perfectly complements to a mutant gene. Then, the mutant gene functions as a primer to initiate branched rolling circle amplification reaction (BRCA),...
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