Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A colorimetric method for point mutation detection using high-fidelity DNA ligase.

Nucleic acids research - 27 Oct 2005

Li Jishan, Chu Xia, Liu Yali, Jiang Jian-Hui, He Zhimin, Zhang Zhiwei, Shen Guoli, Yu Ru-Qin

Abstract excerpt

The present study reported proof-of-principle for a genotyping assay approach that can detect single nucleotide polymorphisms (SNPs) through the gold nanoparticle assembly and the ligase reaction. By incorporating the high-fidelity DNA ligase (Tth DNA ligase) into the allele-specific ligation-based gold nanoparticle assembly, this assay provided a convenient yet powerful colorimetric detection that enabled a...

Topics

Join the communities discussing this publication.

Colorimetry
DNA Ligases
Gold
Nanostructures
Nucleic Acid Denaturation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Nucleic Acid Hybridization

Oligonucleotide Probes

Thionucleotides

Source

Open in PubMed Open full text

Identifiers

PMID: 16257979
DOI: 10.1093/nar/gni163
PMCID: PMC1275593

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A colorimetric method for point mutation detection using high-fidelity DNA ligase.PMID 16257979Read ↗

Combination of DNA ligase reaction and gold nanoparticle-quenched fluorescent oligonucleotides: a simple and efficient approach for fluorescent assaying of single-nucleotide polymorphisms.Semantic proximity Read ↗

A sensitive fluorescence anisotropy method for point mutation detection by using core-shell fluorescent nanoparticles and high-fidelity DNA ligase.Semantic proximity Read ↗

Ligase chain reaction amplification for sensitive electrochemiluminescent detection of single nucleotide polymorphisms.Semantic proximity Read ↗

Colorimetric detection of single-nucleotide polymorphisms with a real-time PCR-like sensitivity.Semantic proximity Read ↗

Advanced Ligase Chain Reaction Strategy to Generate a Circular DNA Walker for Electrochemiluminescent Detection of Single Nucleotide Polymorphism.Semantic proximity Read ↗

Ferrocene-labeled and purification-free electrochemical biosensor based on ligase chain reaction for ultrasensitive single nucleotide polymorphism detection.Semantic proximity Read ↗

Advances in ligase-based nucleic acid amplification technology for detecting gene mutations: a review.Semantic proximity Read ↗

Rolling circle amplification combined with gold nanoparticle aggregates for highly sensitive identification of single-nucleotide polymorphisms.Semantic proximity Read ↗

CRISPR/Cas9 mediated triple signal amplification platform for high selective and sensitive detection of single base mutations.Semantic proximity Read ↗

Controllable Mismatched Ligation for Bioluminescence Screening of Known and Unknown Mutations.Semantic proximity Read ↗

A graphene-based platform for single nucleotide polymorphism (SNP) genotyping.Semantic proximity Read ↗

Detection of single-nucleotide polymorphisms using gold nanoparticles and single-strand-specific nucleases.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.