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Article

Molecular cloning, expression, and characterization of endo-beta-1,4-glucanase genes from Bacillus polymyxa and Bacillus circulans.

Journal of bacteriology - 1 Mar 1990

Baird S D, Johnson D A, Seligy V L

Abstract excerpt

Endo-beta-1,4-glucanase genes from Bacillus circulans and from B. polymyxa were cloned by direct expression by using bacteriophage M13mp9 as the vector. The enzymatic activity of the gene products was detected by using either the Congo red assay or hydroxyethyl cellulose dyed with Ostazin Brilliant Red H-3B. The B. circulans and B. subtilis PAP115 endo-beta-1,4-glucanase genes were shown to be homologous by the...

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Identifiers

PMID: 2307659
DOI: 10.1128/jb.172.3.1576-1586.1990
PMCID: PMC208635

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