Article
Molecular cloning, expression, and characterization of endo-beta-1,4-glucanase genes from Bacillus polymyxa and Bacillus circulans.
Journal of bacteriology - 1 Mar 1990
Baird S D, Johnson D A, Seligy V L
Abstract excerpt
Endo-beta-1,4-glucanase genes from Bacillus circulans and from B. polymyxa were cloned by direct expression by using bacteriophage M13mp9 as the vector. The enzymatic activity of the gene products was detected by using either the Congo red assay or hydroxyethyl cellulose dyed with Ostazin Brillia...
Topics
- Amino Acid Sequence
- Bacillus
- Base Sequence
- Cellulase
- Cloning, Molecular
- Coliphages
- DNA, Bacterial
- Gene Expression
- Genes, Bacterial
- Genotype
- Molecular Sequence Data
- Sequence Homology, Nucleic Acid
- Species Specificity
- Transcription, Genetic