Article
Analysis of the function of a hyperthermophilic endoglucanase from Pyrococcus horikoshii that hydrolyzes crystalline cellulose.
Extremophiles : life under extreme conditions - 1 Feb 2005
Kashima Yasuhiro, Mori Kazushige, Fukada Harumi, Ishikawa Kazuhiko
Abstract excerpt
A hyperthermophilic beta-1,4 endoglucanase was identified in Pyrococcus horikoshii, a hyperthermophilic archaeon. In order to clarify the function of the protein in detail, structural and catalytic site studies were performed using protein engineering. By removing some of the C-terminal sequence of the ORF of the endoglucanase (PH1171), two types of recombinant proteins were expressed from one ORF, using...
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