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Article

Isolation and molecular genetic characterization of the Bacillus subtilis gene (infB) encoding protein synthesis initiation factor 2.

Journal of bacteriology - 1 May 1990

Shazand K, Tucker J, Chiang R, Stansmore K, Sperling-Petersen H U, Grunberg-Manago M, Rabinowitz J C, Leighton T

Abstract excerpt

Western blot (immunoblot) analysis of Bacillus subtilis cell extracts detected two proteins that cross-reacted with monospecific polyclonal antibody raised against Escherichia coli initiation factor 2 alpha (IF2 alpha). Subsequent Southern blot analysis of B. subtilis genomic DNA identified a 1.3-kilobase (kb) HindIII fragment which cross-hybridized with both E. coli and Bacillus stearothermophilus IF2 gene...

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Identifiers

PMID: 2110148
DOI: 10.1128/jb.172.5.2675-2687.1990
PMCID: PMC208912

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