Isolation and molecular genetic characterization of the Bacillus subtilis gene (infB) encoding protein synthesis initiation factor 2.

doi:10.1128/jb.172.5.2675-2687.1990

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Article

Isolation and molecular genetic characterization of the Bacillus subtilis gene (infB) encoding protein synthesis initiation factor 2.

Journal of bacteriology - 1 May 1990

Shazand K, Tucker J, Chiang R, Stansmore K, Sperling-Petersen H U, Grunberg-Manago M, Rabinowitz J C, Leighton T

Abstract excerpt

Western blot (immunoblot) analysis of Bacillus subtilis cell extracts detected two proteins that cross-reacted with monospecific polyclonal antibody raised against Escherichia coli initiation factor 2 alpha (IF2 alpha). Subsequent Southern blot analysis of B. subtilis genomic DNA identified a 1.3...

Topics

Amino Acid Sequence
Antibodies
Bacillus subtilis
Base Sequence
Blotting, Western
Cloning, Molecular
Coliphages
Cross Reactions
DNA, Bacterial
Escherichia coli
Genes, Bacterial

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genetic Complementation Test

Genotype

Molecular Sequence Data

Peptide Initiation Factors

Plasmids

Prokaryotic Initiation Factor-2

Restriction Mapping

Source

Open in PubMed Open full text

Identifiers

PMID: 2110148
DOI: 10.1128/jb.172.5.2675-2687.1990
PMCID: PMC208912

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2110148-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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