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Article

Cloning and expression of the Erwinia carotovora subsp. carotovora gene encoding the low-molecular-weight bacteriocin carocin S1.

Journal of bacteriology - 1 Jan 2007

Chuang Duen-yau, Chien Yung-chei, Wu Huang-Pin

Abstract excerpt

The purpose of this study was to clone the carocin S1 gene and express it in a non-carocin-producing strain of Erwinia carotovora. A mutant, TH22-10, which produced a high-molecular-weight bacteriocin but not a low-molecular-weight bacteriocin, was obtained by Tn5 insertional mutagenesis using H-rif-8-2 (a spontaneous rifampin-resistant mutant of Erwinia carotovora subsp. carotovora 89-H-4). Using thermal...

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Identifiers

PMID: 17071754
DOI: 10.1128/JB.01090-06
PMCID: PMC1797388

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