Bacillopeptidase F of Bacillus subtilis: purification of the protein and cloning of the gene.

doi:10.1128/jb.172.3.1470-1477.1990

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Bacillopeptidase F of Bacillus subtilis: purification of the protein and cloning of the gene.

Journal of bacteriology - 1 Mar 1990

Sloma A, Rufo G A, Rudolph C F, Sullivan B J, Theriault K A, Pero J

Abstract excerpt

We have purified a minor extracellular serine protease from Bacillus subtilis. Characterization of this enzyme indicated that it was most likely the previously reported enzyme bacillopeptidase F. The amino-terminal sequence of the purified protein was determined, and a "guess-mer" oligonucleotide...

Topics

Amino Acid Sequence
Bacillus subtilis
Base Sequence
Blotting, Southern
Chromatography, Affinity
Chromatography, High Pressure Liquid
Chromosome Mapping
Chromosomes, Bacterial
Cloning, Molecular
Genes, Bacterial
Genotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Kinetics

Molecular Sequence Data

Nucleic Acid Hybridization

Oligonucleotide Probes

Plasmids

Restriction Mapping

Sequence Homology, Nucleic Acid

Source

Open in PubMed Open full text

Identifiers

PMID: 2106512
DOI: 10.1128/jb.172.3.1470-1477.1990
PMCID: PMC208622

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2106512-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Bacillopeptidase F of Bacillus subtilis: purification of the protein and cloning of the gene.PMID 2106512Read ↗

Cloning and characterization of the gene for an additional extracellular serine protease of Bacillus subtilis.Semantic proximity Read ↗

Gene encoding a novel extracellular metalloprotease in Bacillus subtilis.Semantic proximity Read ↗

Multiple active forms of a novel serine protease from Bacillus subtilis.Semantic proximity Read ↗

Over‐expression, secretion, biochemical characterisation, and structure analysis of Bacillus subtilis aminopeptidaseSemantic proximity Read ↗

Mapping of the gene for a major penicillin-binding protein to a genetically conserved region of the Bacillus subtilis chromosome and conservation of the protein among related species of Bacillus.Semantic proximity Read ↗

Isolation and molecular genetic characterization of the Bacillus subtilis gene (infB) encoding protein synthesis initiation factor 2.Semantic proximity Read ↗

Cloning, sequencing and genetic mapping of a Bacillus subtilis cell wall hydrolase gene.Semantic proximity Read ↗

Purification and Characterization of YfkN, a Trifunctional Nucleotide Phosphoesterase Secreted by Bacillus SubtilisSemantic proximity Read ↗

Erwinia carotovora subsp. carotovora extracellular protease: characterization and nucleotide sequence of the gene.Semantic proximity Read ↗

Bacillus subtilis alkaline phosphatases III and IV. Cloning, sequencing, and comparisons of deduced amino acid sequence with Escherichia coli alkaline phosphatase three-dimensional structure.Semantic proximity Read ↗

Construction and use of a Bacillus subtilis mutant deficient in multiple protease genes for the expression of eukaryotic genes.Semantic proximity Read ↗

Regulatory Characteristics of Bacillus pumilus Protease Promoters.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.