Article
Bacillopeptidase F of Bacillus subtilis: purification of the protein and cloning of the gene.
Journal of bacteriology - 1 Mar 1990
Sloma A, Rufo G A, Rudolph C F, Sullivan B J, Theriault K A, Pero J
Abstract excerpt
We have purified a minor extracellular serine protease from Bacillus subtilis. Characterization of this enzyme indicated that it was most likely the previously reported enzyme bacillopeptidase F. The amino-terminal sequence of the purified protein was determined, and a "guess-mer" oligonucleotide hybridization probe was constructed on the basis of that sequence. This probe was used to identify and clone the...
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