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Article

Erwinia carotovora subsp. carotovora extracellular protease: characterization and nucleotide sequence of the gene.

Journal of bacteriology - 1 Oct 1991

Kyöstiö S R, Cramer C L, Lacy G H

Abstract excerpt

The prt1 gene encoding extracellular protease from Erwinia carotovora subsp. carotovora EC14 in cosmid pCA7 was subcloned to create plasmid pSK1. The partial nucleotide sequence of the insert in pSK1 (1,878 bp) revealed a 1,041-bp open reading frame (ORF1) that correlated with protease activity in deletion mutants. ORF1 encodes a polypeptide of 347 amino acids with a calculated molecular mass of 38,826 Da....

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Identifiers

PMID: 1917878
DOI: 10.1128/jb.173.20.6537-6546.1991
PMCID: PMC208990

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