Article
Expression of pehA-bla gene fusions in Erwinia carotovora subsp. carotovora and isolation of regulatory mutants affecting polygalacturonase production.
Molecular & general genetics : MGG - 1 Jul 1992
Saarilahti H T, Pirhonen M, Karlsson M B, Flego D, Palva E T
Abstract excerpt
In vitro gene fusions were constructed between the polygalacturonase-encoding pehA gene of the Erwinia carotovora subsp. carotovora (Ecc) strain SCC3193 and the bla gene of pBR322. The gene fusions obtained (75-2, 75-5 and 75-6) encoded hybrid proteins with the entire signal peptide and 70, 260 or 327 amino acids (aa) of the mature 376 aa PehA protein, respectively, fused to the mature part of the periplasmic...
Topics
- Cloning, Molecular
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Genes, Bacterial
- Mutation
- Pectobacterium carotovorum
- Plants, Toxic
- Plasmids
- Polygalacturonase
- Protein Sorting Signals