Article
Mutanase from a Paenibacillus isolate: nucleotide sequence of the gene and properties of recombinant enzymes.
Biochimica et biophysica acta - 1 Apr 2007
Sumitomo Nobuyuki, Saeki Katsuhisa, Ozaki Katsuya, Ito Susumu, Kobayashi Tohru
Abstract excerpt
A mutanase (alpha-1,3-glucanase)-producing microorganism was isolated from a soil sample and was identified as a relative of Paenibacillus sp. The mutanase was purified to homogeneity from culture, and its molecular mass was around 57 kDa. The gene for the mutanase was cloned by PCR using primers based on the N-terminal amino acid sequence of the purified enzyme. The determined nucleotide sequence of the gene...
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