Article
Circular permutation of Bacillus circulans xylanase: a kinetic and structural study.
Biochemistry - 23 Mar 2010
Reitinger Stephan, Yu Ying, Wicki Jacqueline, Ludwiczek Martin, D'Angelo Igor, Baturin Simon, Okon Mark, Strynadka Natalie C J, Lutz Stefan, Withers Stephen G, McIntosh Lawrence P
Abstract excerpt
The 20 kDa Bacillus circulans Bcx is a well-studied endoxylanase with a beta-jellyroll fold that places its N- and C-termini in salt bridge contact. Initial experiments verified that Bcx could be circularly permuted by PCR methods to introduce new termini in loop regions while linking its native...
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