Article
Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant Pol 6.
Applied biochemistry and biotechnology - 1 May 2005
Chaabouni Semia Ellouz, Mechichi Tahar, Limam Ferid, Marzouki Nejib
Abstract excerpt
Two endoglucanases (EGs), EG A and EG B, were purified to homogeneity from Penicillium occitanis mutant Pol 6 culture medium. The molecular weights of EG A and EG B were 31,000 and 28,000 kDa, respectively. The pI was about 3 for EG A and 7.5 for EG B. Optimal activity was obtained at pH 3.5 for both endoglucanases. Optimal temperature for enzyme activity was 60 degrees C for EG A and 50 degrees C for EG B. EG A...
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