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Optimization of ordered plasmid assembly by gap repair in Saccharomyces cerevisiae.

Yeast (Chichester, England) - 1 Aug 2012

Eckert-Boulet Nadine, Pedersen Mette Louise, Krogh Berit Olsen, Lisby Michael

Abstract excerpt

Combinatorial genetic libraries are powerful tools for diversifying and optimizing biomolecules. The process of library assembly is a major limiting factor for library complexity and quality. Gap repair by homologous recombination in Saccharomyces cerevisiae can facilitate in vivo assembly of DNA fragments sharing short patches of sequence homology, thereby supporting generation of high-complexity libraries...

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Identifiers

PMID: 22806834
DOI: 10.1002/yea.2912

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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