Donation: a new, facile method of gene replacement in yeast.

doi:10.1007/BF00282812

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Article

Donation: a new, facile method of gene replacement in yeast.

Molecular & general genetics : MGG - 1 Feb 1993

Roitgrund C, Steinlauf R, Kupiec M

Abstract excerpt

We describe here a new method for the introduction of non-selectable alleles into Saccharomyces cerevisiae, gene replacement by donation. This method only requires the availability of an autonomously replicating, selectable plasmid containing the allele to be introduced into yeast. The plasmid is...

Topics

Genes, Fungal
Mutation
Plasmids
Saccharomyces cerevisiae
Transfection

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