Applications of high efficiency lithium acetate transformation of intact yeast cells using single-stranded nucleic acids as carrier.

doi:10.1002/yea.320070307

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Article

Applications of high efficiency lithium acetate transformation of intact yeast cells using single-stranded nucleic acids as carrier.

Yeast (Chichester, England) - 1 Apr 1991

Gietz R D, Schiestl R H

Abstract excerpt

The highly efficient yeast lithium acetate transformation protocol of Schiestl and Gietz (1989) was tested for its applicability to some of the most important needs of current yeast molecular biology. The method allows efficient cloning of genes by direct transformation of gene libraries into yea...

Topics

Cloning, Molecular
DNA, Fungal
DNA, Single-Stranded
Escherichia coli
Gene Library
Genetic Vectors
Lithium
Mutation
Plasmids
Saccharomyces cerevisiae
Transformation, Genetic

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