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Article

Promoter-trapping in Saccharomyces cerevisiae by radiation-assisted fragment insertion.

Nucleic acids research - 15 Dec 2002

Kiechle Markus, Manivasakam Palaniyandi, Eckardt-Schupp Friederike, Schiestl Robert H, Friedl Anna A

Abstract excerpt

Non-homologous insertion (NHI) of DNA fragments into genomic DNA is a method widely used in insertional mutagenesis screens. In the yeast Saccharomyces cerevisiae, the efficiency of NHI is very low. Here we report that its efficiency can be increased by gamma-irradiation of recipient cells at the time of transformation. Radiation-assisted NHI depends on YKU70, but its efficiency is not improved by inactivation of...

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PMID: 12490727
DOI: 10.1093/nar/gnf136
PMCID: PMC140085

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