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Article

Assembly of designed oligonucleotides as an efficient method for gene recombination: a new tool in directed evolution.

Chembiochem : a European journal of chemical biology - 3 Jan 2003

Zha Dongxing, Eipper Andreas, Reetz Manfred T

Abstract excerpt

A new and practical method for gene recombination with formation of libraries of mutant genes is presented. The method is based on the assembly of appropriately prepared oligonucleotides whose design is guided by sequence information. High recombination frequency with formation of full-length products is achieved by controlled overlapping of the designed oligomers. This process (ADO) minimizes self-hybridization...

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Identifiers

PMID: 12512074
DOI: 10.1002/cbic.200390011

Genes

ADO

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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