Article
Use of gap repair in fission yeast to obtain novel alleles of specific genes.
Nucleic acids research - 15 Oct 1998
Kostrub C F, Lei E P, Enoch T
Abstract excerpt
We have adapted a method for making libraries of mutations in any specific gene for use in the fission yeast Schizosaccharomyces pombe . This elegant and simple method consists of PCR amplification of the gene of interest, followed by co-transformation of fission yeast with the PCR fragment and a linearized plasmid vector prepared such that the ends of the vector share DNA sequence with the ends of the PCR...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.