Use of gap repair in fission yeast to obtain novel alleles of specific genes.

doi:10.1093/nar/26.20.4783

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Article

Use of gap repair in fission yeast to obtain novel alleles of specific genes.

Nucleic acids research - 15 Oct 1998

Kostrub C F, Lei E P, Enoch T

Abstract excerpt

We have adapted a method for making libraries of mutations in any specific gene for use in the fission yeast Schizosaccharomyces pombe . This elegant and simple method consists of PCR amplification of the gene of interest, followed by co-transformation of fission yeast with the PCR fragment and a...

Topics

Alleles
Cell Cycle Proteins
DNA Repair
Gene Library
Genes, Fungal
Mutagenesis
Phenotype
Plasmids
Polymerase Chain Reaction
Recombination, Genetic
Schizosaccharomyces
Schizosaccharomyces pombe Proteins
Transformation, Genetic

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